| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |

Western blot analysis of anti-CD326/EPCAM antibody (BM4250). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCT116- WT whole cell lysates,
Lane 2: human HCT116-EPCAM KO whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD326/EPCAM antigen affinity purified monoclonal antibody (BM4250) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD326/EPCAM at approximately 35-40 kDa. The expected band size for CD326/EPCAM is at 35 kDa.

Western blot analysis of anti-CD326/EPCAM antibody (BM4250). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat small intestine tissue lysates,
Lane 4: mouse small intestine tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD326/EPCAM antigen affinity purified monoclonal antibody (BM4250) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD326/EPCAM at approximately 35-40 kDa. The expected band size for CD326/EPCAM is at 35 kDa.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of human breast tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of human breast tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of mouse small intestine tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of mouse small intestine tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of rat small intestine tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of rat small intestine tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

Immunofluorescent analysis using the Antibody.

Western blot analysis of anti-CD326/EPCAM antibody (BM4250). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCT116- WT whole cell lysates,
Lane 2: human HCT116-EPCAM KO whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD326/EPCAM antigen affinity purified monoclonal antibody (BM4250) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD326/EPCAM at approximately 35-40 kDa. The expected band size for CD326/EPCAM is at 35 kDa.

Western blot analysis of anti-CD326/EPCAM antibody (BM4250). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat small intestine tissue lysates,
Lane 4: mouse small intestine tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD326/EPCAM antigen affinity purified monoclonal antibody (BM4250) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD326/EPCAM at approximately 35-40 kDa. The expected band size for CD326/EPCAM is at 35 kDa.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of human breast tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of human breast tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of mouse small intestine tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of mouse small intestine tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of rat small intestine tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
CD326/EPCAM was detected in a paraffin-embedded section of rat small intestine tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

Immunofluorescent analysis using the Antibody.












