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Anti-CD326/EPCAM Antibody (Clone#CIB-5)

Rabbit monoclonal antibody

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筛选器: All WB IHC ICC/IF

BM4250

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti-CD326/EPCAM antibody (BM4250). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human HCT116- WT whole cell lysates,
    Lane 2: human HCT116-EPCAM KO whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD326/EPCAM antigen affinity purified monoclonal antibody (BM4250) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD326/EPCAM at approximately 35-40 kDa. The expected band size for CD326/EPCAM is at 35 kDa.

    all(13)
  • Western blot analysis of anti-CD326/EPCAM antibody (BM4250). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Caco-2 whole cell lysates,
    Lane 2: human MCF-7 whole cell lysates,
    Lane 3: rat small intestine tissue lysates,
    Lane 4: mouse small intestine tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD326/EPCAM antigen affinity purified monoclonal antibody (BM4250) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD326/EPCAM at approximately 35-40 kDa. The expected band size for CD326/EPCAM is at 35 kDa.

    all(13)
  • IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
    CD326/EPCAM was detected in a paraffin-embedded section of human breast tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(13)
  • IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
    CD326/EPCAM was detected in a paraffin-embedded section of human breast tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(13)
  • IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
    CD326/EPCAM was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(13)
  • IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
    CD326/EPCAM was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(13)
  • IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
    CD326/EPCAM was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(13)
  • IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
    CD326/EPCAM was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(13)
  • IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
    CD326/EPCAM was detected in a paraffin-embedded section of mouse small intestine tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(13)
  • IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
    CD326/EPCAM was detected in a paraffin-embedded section of mouse small intestine tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(13)
  • IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
    CD326/EPCAM was detected in a paraffin-embedded section of rat small intestine tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(13)
  • IHC analysis of CD326/EPCAM using anti-CD326/EPCAM antibody (BM4250) .
    CD326/EPCAM was detected in a paraffin-embedded section of rat small intestine tissue. The tissue section was incubated with rabbit anti-CD326/EPCAM Antibody (BM4250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(13)
  • Immunofluorescent analysis using the Antibody.

    all(13)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称
Anti-CD326/EPCAM Antibody (Clone#CIB-5)
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
CD326; CO 17A; CO17 1A; EGP; EGP 2; EGP314; EGP34; EGP40; Ep CAM; EPCAM; Epithelial glycoprotein; Epithelial glycoprotein 314; ESA; GA733 2; hEGP 2; hEGP314; KS 1/4 antigen; KS1/4; KSA; M1S2; M4S1; MIC18; MK 1; TACST 1; TACSTD1; TROP1
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF
基因名称
EPCAM
克隆号
CIB-5
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthesized peptide derived from human EpCAM
计算分子量
35 kDa
实际分子量
35-40 kDa
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell–cell adhesion in epithelia. This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Uniprot ID
P16422  
文献引用格式
CD326/EPCAM Antibody (Clone#CIB-5) (Boster Biological Technology, Wuhan, China. Catalog#BM4250)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
Epithelial cell adhesion molecule
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-200
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-200

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    Anti-CD326/EPCAM Antibody (Clone#CIB-5)

    筛选器: All WB IHC ICC/IF

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