Anti-EGF Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A00378-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of EGF using anti-EGF antibody (A00378-1).
Lane 1: recombinant rat EGF protein 1ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EGF antigen affinity purified polyclonal antibody (A00378-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EGF at approximately 6 kDa.
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IHC analysis of EGF using anti-EGF antibody (A00378-1).
EGF was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-EGF Antibody (A00378-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of EGF using anti-EGF antibody (A00378-1).
EGF was detected in a paraffin-embedded section of rat small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-EGF Antibody (A00378-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
all(4)

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产品简介>

产品名称

Anti-EGF Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

EGF; HOMG4; Pro epidermal growth factor; URG; Urogastrone

产品类型

Polyclonal

检验物种

rat

应用范围

WB, IHC, ELISA

基因名称

EGF

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived rat EGF recombinant protein (Position: N974-R1026). Rat EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and mouse EGF, respectively.

计算分子量

124 kDa

实际分子量

134 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Uniprot ID

P07522

RRID

AB_2877636

文献引用格式

EGF Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00378-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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