Anti-Serpin C1/Antithrombin III/SERPINC1 Antibody
送常用试剂

Rabbit polyclonal antibody

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筛选器： All WB FCM ELISA

A00469-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti- Antithrombin III/SERPINC1 antibody (A00469-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse testis tissue lysates,
Lane 3: mouse ovary tissue lysates,
Lane 4: mouse liver tissue lysates.
Use rabbit anti- Antithrombin III/SERPINC1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for Antithrombin III/SERPINC1 at approximately 53KD. The expected band size for Antithrombin III/SERPINC1 is at 52KD.
all(3)
Flow Cytometry analysis of HepG2 cells using anti-Serpin C1/Antithrombin III/SERPINC1 antibody (A00469-1).
Overlay histogram showing HepG2 cells stained with A00469-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Serpin C1/Antithrombin III/SERPINC1 Antibody (A00469-1) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
all(3)
all(3)

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产品名称

Anti-Serpin C1/Antithrombin III/SERPINC1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Antithrombin; Antithrombin III; AT3; ATIII; Serpin C1; SERPINC1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, FCM, ELISA

基因名称

SERPINC1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Antithrombin III/SERPINC1 recombinant protein (Position: R89-K464).

计算分子量

53 kDa

实际分子量

53 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Serpin peptidase inhibitor, clade C(antithrombin), member 1, also known as Antithrombin III(AT3) is the most important inhibitor of thrombin OMBIN III and other coagulation proteinases. This gene is mapped to 1q25.1. The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.

Uniprot ID

P01008

文献引用格式

Serpin C1/Antithrombin III/SERPINC1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00469-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

serpin family C member 1

蛋白名全称

Antithrombin-III

实验方案>

实验步骤

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