Anti-IL4R Antibody

Rabbit polyclonal antibody

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筛选器： All IHC FCM ELISA

A00807-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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IHC analysis of IL4R using anti-IL4R antibody (A00807-2).
IL4R was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-IL4R Antibody (A00807-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)
Flow Cytometry analysis of mouse spleen tissue using anti-IL4R antibody (A00807-2).
Overlay histogram showing mouse spleen tissue stained with A00807-2 (Blue line). The tissue was fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IL4R Antibody (A00807-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
all(3)
all(3)

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产品简介>

产品名称

Anti-IL4R Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD124; IL 4 binding protein; IL 4 receptor subunit alpha; IL 4R alpha; IL 4R subunit alpha; IL 4RA; IL4 BP; IL4R; IL-4R; IL 4R; IL4RA; interleukin 4 receptor; sIL4Ralpha/prot; Soluble IL 4R alpha

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

IHC, FCM, ELISA

基因名称

Il4r

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived rat IL4R recombinant protein (Position: I26-E719).

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

IL4R(INTERLEUKIN 4 RECEPTOR) is a human gene that encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. The IL4R gene is localized to 16p12.1-p11.2 by in situ hybridization and Southern blot analysis of DNA from a panel of mouse-human somatic cell lines. IL4 plays a major role in immunoglobulin E(IgE) production. Its signal is conferred to effector cells through binding to the alpha chain of the IL4 receptor. IL4 interacts with IL4R with high affinity, leading to dimerization with either the common gamma chain,a component of receptors for a number of cytokines, to create a type I receptor, or with IL13RA1 to form a type II receptor.The IL4R gene contains 12 exons and IL4R is a complex of at least 2 components, one of which is a novel affinity converting subunit that is critical for cellular signal transduction. See also IL13RA1 and IL13RA2.

Uniprot ID

Q63257

RRID

AB_3081247

文献引用格式

IL4R Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00807-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

interleukin 4 receptor

蛋白名全称

Interleukin-4 receptor subunit alpha

实验方案>

实验步骤

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