Anti-IL4R Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA0984

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of IL4R using anti-IL4R antibody (BA0984). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A431 whole cell lysates,
Lane 2: SMMC whole cell lysates,
Lane 3: U87 whole cell lysates,
Lane 4: 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL4R antigen affinity purified polyclonal antibody (BA0984) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL4R at approximately 90 kDa. The expected band size for IL4R is at 90 kDa.
all(2)
IHC analysis of IL4R using anti-IL4R antibody (BA0984).
IL4R was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-IL4R Antibody (BA0984) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品名称

Anti-IL4R Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD124; IL 4 binding protein; IL 4 receptor subunit alpha; IL 4R alpha; IL 4R subunit alpha; IL 4RA; IL4 BP; IL4R; IL-4R; IL 4R; IL4RA; interleukin 4 receptor; sIL4Ralpha/prot; Soluble IL 4R alpha

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

IL4R

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human IL4R.

计算分子量

90 kDa

实际分子量

90 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

IL4R(INTERLEUKIN 4 RECEPTOR) is a human gene that encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. The IL4R gene is localized to 16p12.1-p11.2 by in situ hybridization and Southern blot analysis of DNA from a panel of mouse-human somatic cell lines. IL4 plays a major role in immunoglobulin E(IgE) production. Its signal is conferred to effector cells through binding to the alpha chain of the IL4 receptor. IL4 interacts with IL4R with high affinity, leading to dimerization with either the common gamma chain,a component of receptors for a number of cytokines, to create a type I receptor, or with IL13RA1 to form a type II receptor.The IL4R gene contains 12 exons and IL4R is a complex of at least 2 components, one of which is a novel affinity converting subunit that is critical for cellular signal transduction. See also IL13RA1 and IL13RA2.

Uniprot ID

P24394

文献引用格式

IL4R Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0984)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

interleukin 4 receptor

蛋白名全称

Interleukin-4 receptor subunit alpha(IL-4 receptor subunit alpha/IL-4R subunit alpha/IL-4R-alpha/IL-4RA)

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实验步骤

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