Western blot (WB): | 1:500-2000 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Western blot analysis of TGFBR1 using anti-TGFBR1 antibody (A00832). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HepG2 tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TGFBR1 antigen affinity purified polyclonal antibody (A00832) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TGFBR1 at approximately 56 kDa. The expected band size for TGFBR1 is at 56 kDa.
Western blot analysis of TGFBR1 using anti-TGFBR1 antibody (A00832). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HepG2 tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TGFBR1 antigen affinity purified polyclonal antibody (A00832) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TGFBR1 at approximately 56 kDa. The expected band size for TGFBR1 is at 56 kDa.