Anti-NEDD4 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

A00984-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of NEDD4 using anti-NEDD4 antibody (A00984-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human MDA-MB-453 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NEDD4 antigen affinity purified polyclonal antibody (A00984-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NEDD4 at approximately 105-150 kDa. The expected band size for NEDD4 is at 149 kDa .
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产品简介>

产品名称

Anti-NEDD4 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

KIAA0093; NEDD 4; NEDD4; NEDD4 1; RPF1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ELISA

基因名称

NEDD4

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human NEDD4 recombinant protein (Position: N960-F1245).

计算分子量

149 kDa

实际分子量

105-150 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

E3 ubiquitin-protein ligase NEDD4, also known as neural precursor cell expressed developmentally down-regulated protein 4 (NEDD4), is an enzyme that in humans is encoded by the NEDD4 gene. This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN.

Uniprot ID

P46934

文献引用格式

NEDD4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00984-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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