Anti-NEDD4 Antibody (Clone#AEGB-14)

Rabbit monoclonal antibody

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筛选器： All WB IHC IP FCM

BM5598

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-NEDD4 antibody (BM5598). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse Neuro-2a whole cell lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NEDD4 antigen affinity purified monoclonal antibody (BM5598) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NEDD4 at approximately 130 kDa. The expected band size for NEDD4 is at 149 kDa.
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IHC analysis of NEDD4 using anti-NEDD4 antibody (BM5598) .
NEDD4 was detected in a paraffin-embedded section of human bladder cancer tissue. The tissue section was incubated with rabbit anti-NEDD4 Antibody (BM5598) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of NEDD4 using anti-NEDD4 antibody (BM5598) .
NEDD4 was detected in a paraffin-embedded section of human pancreas cancer tissue. The tissue section was incubated with rabbit anti-NEDD4 Antibody (BM5598) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of NEDD4 using anti-NEDD4 antibody (BM5598) .
NEDD4 was detected in a paraffin-embedded section of human pancreas cancer tissue. The tissue section was incubated with rabbit anti-NEDD4 Antibody (BM5598) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IP analysis of NEDD4 using anti-NEDD4 antibody (BM5598) in A549 whole cell lysate.
Western blot analysis of NEDD4 using anti- NEDD4 antibody (BM5598).
Lane 1: A549 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- NEDD4 antibody in A549 whole cell lysate,
Lane 3: anti- NEDD4 antibody (2μg) + A549 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- NEDD4 antigen affinity purified monoclonal antibody (BM5598) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NEDD4 at approximately 135 kDa. The expected band size for NEDD4 is at 150 kDa.
all(6)
all(6)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-NEDD4 Antibody (Clone#AEGB-14)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

KIAA0093; NEDD 4; NEDD4; NEDD4 1; RPF1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IP, FCM

基因名称

NEDD4

克隆号

AEGB-14

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human NEDD4 E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity.

计算分子量

105-150 kDa

实际分子量

135 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

E3 ubiquitin-protein ligase NEDD4, also known as neural precursor cell expressed developmentally down-regulated protein 4 (NEDD4), is an enzyme that in humans is encoded by the NEDD4 gene. This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN.

Uniprot ID

P46934

文献引用格式

NEDD4 Antibody (Clone#AEGB-14) (Boster Biological Technology, Wuhan, China. Catalog#BM5598)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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