Anti-ALPL Antibody

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF ELISA

A01008

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ALPL using anti-ALPL antibody (A01008). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Human Hela whole cell lysates,
Lane 2: Human HEK293 whole cell lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: Rat RH35 whole cell lysates,
Lane 5: Mouse kidney tissue lysates,
Lane 6: Mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ALPL antigen affinity purified polyclonal antibody (A01008) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ALPL at approximately 57/80 kDa. The expected band size for ALPL is at 57 kDa.
all(3)
IF analysis of ALPL using anti-ALPL antibody (A01008).
ALPL was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ALPL Antibody (A01008) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(3)
all(3)

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产品名称

Anti-ALPL Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Alkaline phosphatase; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase tissue nonspecific isozyme; ALPL; BLAP; Bone Alkaline Phosphatase; NBAP

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF, ELISA

基因名称

ALPL

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Alkaline phosphatase/ALPL recombinant protein (Position: K229-H482).

计算分子量

57 kDa

实际分子量

57/80 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Alkaline phosphatase(ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific(found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts ,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine(PEA) and pyridoxal-5-prime-phosphate(PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Uniprot ID

P05186

RRID

AB_3081313

文献引用格式

ALPL Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01008)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

alkaline phosphatase, biomineralization associated

蛋白名全称

Alkaline phosphatase, tissue-nonspecific isozyme

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