Anti-ALPL Antibody (Clone#DBF-1)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM4284

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-ALPL antibody (BM4284). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ALPL antigen affinity purified monoclonal antibody (BM4284) at a dilution of 1:2000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ALPL at approximately 80 kDa. The expected band size for ALPL is at 57 kDa.
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Immunohistochemical analysis of paraffin-embedded human liver, using Alkaline Phosphatase Antibody.
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Immunofluorescent analysis of Hela cells, using Alkaline Phosphatase Antibody .
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产品名称

Anti-ALPL Antibody (Clone#DBF-1)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Alkaline phosphatase; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase tissue nonspecific isozyme; ALPL; BLAP; Bone Alkaline Phosphatase; NBAP

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

ALPL

克隆号

DBF-1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Alkaline Phosphatase

计算分子量

57 kDa

实际分子量

80 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Alkaline phosphatase(ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific(found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts ,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine(PEA) and pyridoxal-5-prime-phosphate(PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Uniprot ID

P05186

文献引用格式

ALPL Antibody (Clone#DBF-1) (Boster Biological Technology, Wuhan, China. Catalog#BM4284)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Alkaline phosphatase, tissue-nonspecific isozyme

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