Anti-CHAT Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A01192-5

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CHAT using anti-CHAT antibody (A01192-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CHAT antigen affinity purified polyclonal antibody (A01192-5) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CHAT at approximately 83 kDa. The expected band size for CHAT is at 83 kDa.
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IHC analysis of CHAT using anti-CHAT antibody (A01192-5).
CHAT was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CHAT Antibody (A01192-5) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of CHAT using anti-CHAT antibody (A01192-5).
CHAT was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CHAT Antibody (A01192-5) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
all(4)

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产品简介>

产品名称

Anti-CHAT Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CHAT; CHOACTase; Choline acetylase; choline acetyltransferase; Choline O acetyltransferase; CMS1A; CMS1A2

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC, ELISA

基因名称

CHAT

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived rat Choline Acetyltransferase/Chat recombinant protein (Position: E19-D612).

计算分子量

83 kDa

实际分子量

83 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Choline acetyltransferase (commonly abbreviated as°ChAT,but sometimes°CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. In humans,the choline acetyltransferase enzyme is encoded by the°CHAT gene. This gene product is a characteristic feature of cholinergic neurons,and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene,and some of these variants have been shown to encode more than one isoform.

Uniprot ID

P32738

RRID

AB_3081372

文献引用格式

CHAT Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01192-5)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

choline O-acetyltransferase

蛋白名全称

Choline O-acetyltransferase

实验方案>

实验步骤

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