Anti-Thioredoxin/TXN Antibody
送常用试剂

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF ELISA

A01219-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Thioredoxin TRX antibody (A01219-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat small intestine tissue lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse small intestine tissue lysates,
Lane 8: mouse lung tissue lysates,
Lane 9: mouse heart tissue lysates,
Lane 10: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Thioredoxin TRX antigen affinity purified polyclonal antibody (A01219-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Thioredoxin TRX at approximately 12 kDa. The expected band size for Thioredoxin TRX is at 12 kDa.
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IHC analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody (A01219-1).
Thioredoxin TRX was detected in a paraffin-embedded section of mouse small intestine tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody (A01219-1).
Thioredoxin TRX was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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ICC analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody (A01219-1).
Thioredoxin TRX was detected in an immunocytochemical section of LLC cells. The section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
ICC/IF analysis of Thioredoxin TRX using anti-Thioredoxin TRX antibody (A01219-1).
Thioredoxin TRX was detected in an immunocytochemical section of NIH/3T3 cells. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(6)
all(6)

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产品简介>

产品名称

Anti-Thioredoxin/TXN Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

ADF; ATL derived factor; SASP; Thioredoxin; TRDX; TRX; TRX1; TXN

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC, ICC/IF, ELISA

基因名称

Txn

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived mouse Thioredoxin TRX recombinant protein (Position: V2-A105).

计算分子量

12 kDa

实际分子量

12 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Thioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

P10639

RRID

AB_3081387

文献引用格式

Thioredoxin/TXN Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01219-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

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