Anti-Occludin/OCLN Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF IP

A01246-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti-Occludin/OCLN antibody (A01246-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Occludin/OCLN antigen affinity purified polyclonal antibody (A01246-4) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Occludin/OCLN at approximately 59 kDa. The expected band size for Occludin/OCLN is at 59 kDa.
all(6)
IHC analysis of Occludin/OCLN using anti-Occludin/OCLN antibody (A01246-4).
Occludin/OCLN was detected in a paraffin-embedded section of human stomach tissue. The tissue section was incubated with rabbit anti-Occludin/OCLN Antibody (A01246-4) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of Occludin/OCLN using anti-Occludin/OCLN antibody (A01246-4).
Occludin/OCLN was detected in a paraffin-embedded section of human stomach tissue. The tissue section was incubated with rabbit anti-Occludin/OCLN Antibody (A01246-4) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of Occludin/OCLN using anti-Occludin/OCLN antibody (A01246-4).
Occludin/OCLN was detected in a paraffin-embedded section of human stomach tissue. The tissue section was incubated with rabbit anti-Occludin/OCLN Antibody (A01246-4) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
all(6)
IP analysis of Occludin/OCLN using anti-Occludin/OCLN antibody (A01246-4) in HepG2 whole cell lysate.
Western blot analysis of Occludin/OCLN using anti- Occludin/OCLN antibody (A01246-4).
Lane 1: HepG2 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- Occludin/OCLN antibody in HepG2 whole cell lysate,
Lane 3: anti- Occludin/OCLN antibody (2μg) + HepG2 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- Occludin/OCLN antigen affinity purified polyclonal antibody (A01246-4) at a dilution of 1:1000 and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Light Chain Specific). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Occludin/OCLN at approximately 59 kDa. The expected band size for Occludin/OCLN is at 59 kDa.
all(6)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Occludin/OCLN Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Occludin; OCLN

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP

基因名称

OCLN

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Occludin/OCLN recombinant protein (Position: S358-Q520). Human Occludin/OCLN shares 88.8% and 88.2% amino acid (aa) sequence identity with mouse and rat Occludin/OCLN, respectively.

计算分子量

59 kDa

实际分子量

59 kDa

成分

500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Occludin is an integral membrane protein that is located at tight junctions. It is a member of a family of proteins containing the highly conserved Marvel domain, which contains 4 transmembrane-helix regions. This gene is mapped to 5q13.2. Occludin regulates TGF-beta receptor type I localization for efficient TGF-beta-dependent dissolution of tight junctions during epithelial-mesenchymal transitions. Human Occludin is an essential hepatitis C virus (HCV) cell entry factor that is able to render murine cells infectable with HCV glycoproteins. It has been found that occludin is involved in cell migration and functions to recruit active PI3 kinase to the leading edge, resulting in RAC1 activation and formation of lamellipodia.

Uniprot ID

Q16625

Gene ID

100506658

文献引用格式

Occludin/OCLN Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01246-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

occludin

蛋白名全称

Occludin

实验方案>

实验步骤

引用文献(0)>

清除