Anti-CD16/FCGR3A Antibody

Rabbit polyclonal antibody

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筛选器： All IHC ICC/IF FCM ELISA

A01408-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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IHC analysis of CD16/FCGR3A using anti-CD16/FCGR3A antibody (A01408-1).
CD16/FCGR3A was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD16/FCGR3A Antibody (A01408-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CD16/FCGR3A using anti-CD16/FCGR3A antibody (A01408-1).
CD16/FCGR3A was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD16/FCGR3A Antibody (A01408-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CD16/FCGR3A using anti-CD16/FCGR3A antibody (A01408-1).
CD16/FCGR3A was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD16/FCGR3A Antibody (A01408-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of CD16/FCGR3A using anti-CD16/FCGR3A antibody (A01408-1).
CD16/FCGR3A was detected in an immunocytochemical section of K562 cells. The section was incubated with rabbit anti-CD16/FCGR3A Antibody (A01408-1) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of HL-60 cells using anti-CD16/FCGR3A antibody (A01408-1).
Overlay histogram showing HL-60 cells stained with A01408-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD16/FCGR3A Antibody (A01408-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品名称

Anti-CD16/FCGR3A Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD16; CD16A; CD16a antigen; Fc gamma RIII; Fc gamma RIII alpha; Fc gamma RIIIa; FCG3; FCGR3; FCGR3A; FCGRIII; FCR 10; FCRIII; FCRIIIA; IGFR3; IgG Fc receptor III 2

产品类型

Polyclonal

检验物种

human

应用范围

IHC, ICC/IF, FCM, ELISA

基因名称

FCGR3A

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human CD16 recombinant protein (Position: Q101-D166). Human CD16 shares 50.9% and 56.5% amino acid (aa) sequence identity with mouse and rat CD16, respectively.

实际分子量

45KD

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Low affinity immunoglobulin gamma Fc region receptor III-A is a protein that in humans is encoded by the FCGR3A gene. This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

P08637

文献引用格式

CD16/FCGR3A Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01408-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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