Anti-CD16/FCGR3A Antibody (Clone#OTI2A2)

Mouse monoclonal antibody

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筛选器： All WB IHC FCM

M01408-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR3A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR3A.(1:1000)
all(5)
IHC analysis of CD16/FCGR3A using anti-CD16/FCGR3A antibody (M01408-2).
CD16/FCGR3A was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with mouse anti-CD16/FCGR3A Antibody (M01408-2) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of CD16/FCGR3A using anti-CD16/FCGR3A antibody (M01408-2).
CD16/FCGR3A was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with mouse anti-CD16/FCGR3A Antibody (M01408-2) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of CD16/FCGR3A using anti-CD16/FCGR3A antibody (M01408-2).
CD16/FCGR3A was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with mouse anti-CD16/FCGR3A Antibody (M01408-2) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
all(5)
Flow cytometric analysis of living 293T cells transfected with FCGR3A overexpression plasmid , Red)/empty vector (Blue) using anti-FCGR3A antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control.（1：100）
all(5)

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产品简介>

产品名称

Anti-CD16/FCGR3A Antibody (Clone#OTI2A2)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD16; CD16A; CD16a antigen; Fc gamma RIII; Fc gamma RIII alpha; Fc gamma RIIIa; FCG3; FCGR3; FCGR3A; FCGRIII; FCR 10; FCRIII; FCRIIIA; IGFR3; IgG Fc receptor III 2

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, FCM

基因名称

FCGR3A

克隆号

OTI2A2

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human FCGR3A (NP_001121065) produced in HEK293T cell.

计算分子量

29 kDa

实际分子量

29.09 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.

背景资料

This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

Uniprot ID

P08637

文献引用格式

CD16/FCGR3A Antibody (Clone#OTI2A2) (Boster Biological Technology, Wuhan, China. Catalog#M01408-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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