Anti-eNOS/NOS3 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM ELISA

A01604-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of eNOS/NOS3 using anti-eNOS/NOS3 antibody (A01604-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse kidne tissue lysates,
Lane 7: mouse small intestine tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-eNOS/NOS3 antigen affinity purified polyclonal antibody (A01604-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for eNOS/NOS3 at approximately 133 kDa. The expected band size for eNOS/NOS3 is at 133 kDa.
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IHC analysis of eNOS/NOS3 using anti-eNOS/NOS3 antibody (A01604-2).
eNOS/NOS3 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-eNOS/NOS3 Antibody (A01604-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of eNOS/NOS3 using anti-eNOS/NOS3 antibody (A01604-2).
eNOS/NOS3 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-eNOS/NOS3 Antibody (A01604-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of eNOS/NOS3 using anti-eNOS/NOS3 antibody (A01604-2).
eNOS/NOS3 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-eNOS/NOS3 Antibody (A01604-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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Flow Cytometry analysis of HepG2 cells using anti-eNOS/NOS3 antibody (A01604-2).
Overlay histogram showing HepG2 cells stained with A01604-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-eNOS/NOS3 Antibody (A01604-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-eNOS/NOS3 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

cNOS; Constitutive NOS; EC NOS; ECNOS; Endothelial NOS; eNOS; NOS type III; NOS3; NOSIII

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM, ELISA

基因名称

NOS3

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human eNOS/NOS3 recombinant protein (Position: Q1052-P1203). Human NOS3 shares 91.2% and 90.6% amino acid (aa) sequence identity with mouse and rat NOS3, respectively.

计算分子量

133 kDa

实际分子量

133 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

NOS3(Nitric Oxide Synthase 3), also called ENOS, a nitric oxide synthase that generates NO in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle°Contraction and platelet aggregation. The NOS3 gene is mapped on 7q36.1. Variations in this gene are associated with susceptibility to°Coronary spasm. Fulton et al. (1999) concluded the eNOS is an AKT substrate linking signal transduction by AKT to the release of the gaseous second messenger nitric oxide. AKT mediates the activation of eNOS, leading to increased nitric oxide production. Inhibition of the PI3K AKT pathway or mutation of the AKT site on eNOS protein at serine-1177 attenuated the serine phosphorylation and prevented the activation of eNOS. RT-PCR analysis showed that expression of NOS3 in human umbilical vein endothelial cells (HUVECs) and human aortic vascular smooth muscle cells (HAOVSMCs) was inversely proportional to that of NOS3AS.

Uniprot ID

P29474

RRID

AB_3081463

文献引用格式

eNOS/NOS3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01604-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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