Anti-eNOS/NOS3 Antibody

筛选器： All WB ICC/IF FCM ELISA

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  Western blot analysis of anti- eNOS/NOS3 antibody (A01604-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates.
  Use rabbit anti- eNOS/NOS3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for eNOS/NOS3 at approximately 140KD. The expected band size for eNOS/NOS3 is at 133KD.

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  IF analysis of eNOS/NOS3 using anti-eNOS/NOS3 antibody (A01604-3).
  eNOS/NOS3 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-eNOS/NOS3 Antibody (A01604-3) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of U937 cells using anti-eNOS/NOS3 antibody (A01604-3).
  Overlay histogram showing U937 cells stained with A01604-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-eNOS/NOS3 Antibody (A01604-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of anti- eNOS/NOS3 antibody (A01604-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates.
  Use rabbit anti- eNOS/NOS3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for eNOS/NOS3 at approximately 140KD. The expected band size for eNOS/NOS3 is at 133KD.

• 

  IF analysis of eNOS/NOS3 using anti-eNOS/NOS3 antibody (A01604-3).
  eNOS/NOS3 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-eNOS/NOS3 Antibody (A01604-3) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of U937 cells using anti-eNOS/NOS3 antibody (A01604-3).
  Overlay histogram showing U937 cells stained with A01604-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-eNOS/NOS3 Antibody (A01604-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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