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Anti-Trefoil factor 3/TFF3 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ELISA

A01738-2

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of Trefoil factor 3/TFF3 using anti-Trefoil factor 3/TFF3 antibody (A01738-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat gaster tissue lysates,
    Lane 2: rat small intestine tissue lysates,
    Lane 3: mouse small intestine tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Trefoil factor 3/TFF3 antigen affinity purified polyclonal antibody (A01738-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Trefoil factor 3/TFF3 at approximately 9 kDa. The expected band size for Trefoil factor 3/TFF3 is at 9 kDa.

    all(6)
  • IHC analysis of Trefoil factor 3/TFF3 using anti-Trefoil factor 3/TFF3 antibody (A01738-2).
    Trefoil factor 3/TFF3 was detected in a paraffin-embedded section of mouse gaster tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Trefoil factor 3/TFF3 Antibody (A01738-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(6)
  • IHC analysis of Trefoil factor 3/TFF3 using anti-Trefoil factor 3/TFF3 antibody (A01738-2).
    Trefoil factor 3/TFF3 was detected in a paraffin-embedded section of mouse small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Trefoil factor 3/TFF3 Antibody (A01738-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(6)
  • IHC analysis of Trefoil factor 3/TFF3 using anti-Trefoil factor 3/TFF3 antibody (A01738-2).
    Trefoil factor 3/TFF3 was detected in a paraffin-embedded section of rat gaster tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Trefoil factor 3/TFF3 Antibody (A01738-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(6)
  • IHC analysis of Trefoil factor 3/TFF3 using anti-Trefoil factor 3/TFF3 antibody (A01738-2).
    Trefoil factor 3/TFF3 was detected in a paraffin-embedded section of rat small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Trefoil factor 3/TFF3 Antibody (A01738-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(6)

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产品简介>

产品名称
Anti-Trefoil factor 3/TFF3 Antibody
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
HITF; hP1.B; Intestinal trefoil factor; ITF; Polypeptide P1.B; TFF3; TFI; Trefoil factor 3; trefoil factor 3 (intestinal)
产品类型
Polyclonal
检验物种
mouse, rat
应用范围
WB, IHC, ELISA
基因名称
Tff3
宿主
Rabbit
抗体亚型
IgG
免疫原
E. coli-derived rat TFF3 recombinant protein (Position: Q23-F81).
计算分子量
9 kDa
实际分子量
9 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.
Uniprot ID
Q03191  
文献引用格式
Trefoil factor 3/TFF3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01738-2)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
ELISA:1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Trefoil factor 3/TFF3 Antibody

    筛选器: All WB IHC ELISA

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