| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| ImmunoPrecipitation (IP): | 1:30 |

Western blot analysis of anti-Trefoil factor 3/TFF3 antibody (BM5331). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Trefoil factor 3/TFF3 antigen affinity purified monoclonal antibody (BM5331) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Trefoil factor 3/TFF3 at approximately 13 kDa. The expected band size for Trefoil factor 3/TFF3 is at 9 kDa.

Western blot analysis of anti-Trefoil factor 3/TFF3 antibody (BM5331). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Trefoil factor 3/TFF3 antigen affinity purified monoclonal antibody (BM5331) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Trefoil factor 3/TFF3 at approximately 13 kDa. The expected band size for Trefoil factor 3/TFF3 is at 9 kDa.




