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Anti-PTBP1 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ICC/IF FCM ELISA

A01798

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of PTBP1 using anti-PTBP1 antibody (A01798). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human K562 whole cell lysates,
    Lane 2: human A431 whole cell lysates,
    Lane 3: human A549 whole cell lysates,
    Lane 4: human 293T whole cell lysates,
    Lane 5: rat testis tissue lysates,
    Lane 6: mouse testis tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PTBP1 antigen affinity purified polyclonal antibody (A01798) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PTBP1 at approximately 57 kDa. The expected band size for PTBP1 is at 57 kDa.

    all(11)
  • Western blot analysis of PTBP1 using anti-PTBP1 antibody (A01798). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human A431 whole cell lysates,
    Lane 2: human RT4 whole cell lysates,
    Lane 3: human SiHa whole cell lysates,
    Lane 4: human HepG2 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PTBP1 antigen affinity purified polyclonal antibody (A01798) at a dilution of 1:1000 and probed with a DyLight 647 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1150). A specific band was detected for PTBP1 at approximately 57 kDa. The expected band size for PTBP1 is at 57 kDa.

    all(11)
  • IHC analysis of PTBP1 using anti-PTBP1 antibody (A01798).
    PTBP1 was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PTBP1 Antibody (A01798) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IHC analysis of PTBP1 using anti-PTBP1 antibody (A01798).
    PTBP1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PTBP1 Antibody (A01798) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IHC analysis of PTBP1 using anti-PTBP1 antibody (A01798).
    PTBP1 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PTBP1 Antibody (A01798) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IHC analysis of PTBP1 using anti-PTBP1 antibody (A01798).
    PTBP1 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PTBP1 Antibody (A01798) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IHC analysis of PTBP1 using anti-PTBP1 antibody (A01798).
    PTBP1 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PTBP1 Antibody (A01798) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IHC analysis of PTBP1 using anti-PTBP1 antibody (A01798).
    PTBP1 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PTBP1 Antibody (A01798) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IF analysis of PTBP1 using anti- PTBP1 antibody (A01798).
    PTBP1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti- PTBP1 Antibody (A01798) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

    all(11)
  • Flow Cytometry analysis of 293T cells using anti-PTBP1 antibody (A01798).
    Overlay histogram showing 293T cells stained with A01798 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTBP1 Antibody (A01798) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

    all(11)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称
Anti-PTBP1 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
HNRNP I; HNRNPI; HNRPI; pPTB; PTB; PTB 1; PTB T; PTB2; PTB3; PTB4; PTBP1
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, FCM, ELISA
基因名称
PTBP1
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived human PTBP1 recombinant protein (Position: M1-A504).
计算分子量
57 kDa
实际分子量
57 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Polypyrimidine tract-binding protein 1 is a protein that in humans is encoded by the PTBP1 gene. It is mapped to 19p13.3. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
Uniprot ID
P26599  
RRID
文献引用格式
PTBP1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01798)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-400
Flow Cytometry (Fixed):1:50-200
Enzyme linked immunosorbent assay (ELISA):1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-PTBP1 Antibody

    筛选器: All WB IHC ICC/IF FCM ELISA

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