Anti-Beta Galactosidase/GLB1 Antibody
送常用试剂

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM ELISA

A01829-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti- GLB1 antibody (A01829-2). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
Use rabbit anti- GLB1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for GLB1 at approximately 65-85KD. The expected band size for GLB1 is at 76KD.
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IHC analysis of Beta Galactosidase/GLB1 using anti-Beta Galactosidase/GLB1 antibody (A01829-2).
Beta Galactosidase/GLB1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-Beta Galactosidase/GLB1 Antibody (A01829-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Beta Galactosidase/GLB1 using anti-Beta Galactosidase/GLB1 antibody (A01829-2).
Beta Galactosidase/GLB1 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-Beta Galactosidase/GLB1 Antibody (A01829-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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ICC/IF analysis of Beta Galactosidase/GLB1 using anti-Beta Galactosidase/GLB1 antibody (A01829-2).
Beta Galactosidase/GLB1 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-Beta Galactosidase/GLB1 Antibody (A01829-2) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of MCF-7 cells using anti-Beta Galactosidase/GLB1 antibody (A01829-2).
Overlay histogram showing MCF-7 cells stained with A01829-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Beta Galactosidase/GLB1 Antibody (A01829-2) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介>

产品名称

Anti-Beta Galactosidase/GLB1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Acid beta galactosidase; Beta galactosidase; EBP; Elastin receptor 1; ELNR1; galactosidase; beta 1; GLB1; Lactase

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, FCM, ELISA

基因名称

GLB1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human GLB1/Beta-galactosidase recombinant protein (Position: Q46-K655).

计算分子量

76 kDa

实际分子量

65-85 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Galactosidase, beta 1, also known as GLB1, is a protein which in humans is encoded by the GLB1 gene. I t is mapped to 3p22.3. This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome.

Uniprot ID

P16278

文献引用格式

Beta Galactosidase/GLB1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01829-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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