Anti-PKC Beta/PRKCB Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC FCM

A01940

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of PKC Beta/PRKCB using anti-PKC Beta/PRKCB antibody (A01940). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKC Beta/PRKCB antigen affinity purified polyclonal antibody (A01940) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PKC Beta/PRKCB at approximately 77 kDa. The expected band size for PKC Beta/PRKCB is at 77 kDa.
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IHC analysis of PKC Beta/PRKCB using anti-PKC Beta/PRKCB antibody (A01940) .
PKC Beta/PRKCB was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-PKC Beta/PRKCB Antibody (A01940) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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Flow Cytometry analysis of Jurkat cells using anti-PKC Beta/PRKCB antibody (A01940).
Overlay histogram showing Jurkat cells stained with A01940 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC Beta/PRKCB Antibody (A01940) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
all(3)

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产品简介>

产品名称

Anti-PKC Beta/PRKCB Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

PKC B; PKC beta; PKCB; PRKCB; PRKCB1; PRKCB2; Protein kinase C beta type; protein kinase C; beta

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM

基因名称

PRKCB

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human PKC beta 1/PRKCB, identical to the related mouse and rat sequences.

计算分子量

77 kDa

实际分子量

77 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Protein kinase C beta type is an enzyme that in humans is encoded by the PRKCB gene. It is a member of the protein kinase C (PKC) gene family. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. It has been found that PRKCB activated by oxidative conditions in the cell, induces phosphorylation of p66(SHC) and triggers mitochondrial accumulation of the protein after it is recognized by the prolyl isomerase PIN1.

Uniprot ID

P05771

文献引用格式

PKC Beta/PRKCB Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01940)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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