Anti-PKC Beta/PRKCB Antibody (Clone#DOC-16)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM4261

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-PKC Beta/PRKCB antibody (BM4261). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKC Beta/PRKCB antigen affinity purified monoclonal antibody (BM4261) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PKC Beta/PRKCB at approximately 77 kDa. The expected band size for PKC Beta/PRKCB is at 77 kDa.
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产品简介>

产品名称

Anti-PKC Beta/PRKCB Antibody (Clone#DOC-16)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

PKC B; PKC beta; PKCB; PRKCB; PRKCB1; PRKCB2; Protein kinase C beta type; protein kinase C; beta

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

PRKCB

克隆号

DOC-16

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human PKC beta 2

计算分子量

77 kDa

实际分子量

77 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Protein kinase C beta type is an enzyme that in humans is encoded by the PRKCB gene. It is a member of the protein kinase C (PKC) gene family. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. It has been found that PRKCB activated by oxidative conditions in the cell, induces phosphorylation of p66(SHC) and triggers mitochondrial accumulation of the protein after it is recognized by the prolyl isomerase PIN1.

Uniprot ID

P05771

文献引用格式

PKC Beta/PRKCB Antibody (Clone#DOC-16) (Boster Biological Technology, Wuhan, China. Catalog#BM4261)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Protein kinase C beta type

实验方案>

实验步骤

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