Western blot (WB): | 1:500-2000 |
ELISA: | 1:100-1000 |
Western blot analysis of FAT10/UBD using anti-FAT10/UBD antibody (A01970-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FAT10/UBD antigen affinity purified polyclonal antibody (A01970-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FAT10/UBD at approximately 18 kDa. The expected band size for FAT10/UBD is at 18 kDa.
Western blot analysis of FAT10/UBD using anti-FAT10/UBD antibody (A01970-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FAT10/UBD antigen affinity purified polyclonal antibody (A01970-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FAT10/UBD at approximately 18 kDa. The expected band size for FAT10/UBD is at 18 kDa.