Anti-FAT10/UBD Antibody (Clone#HID-21)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4765

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-FAT10/UBD antibody (BM4765). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: human SH-SY5Y whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FAT10/UBD antigen affinity purified monoclonal antibody (BM4765) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FAT10/UBD at approximately 23 kDa. The expected band size for FAT10/UBD is at 18 kDa.
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产品简介>

产品名称

Anti-FAT10/UBD Antibody (Clone#HID-21)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Diubiquitin; FAT10; GABBR1; UBD; UBD 3; ubiquitin D; Ubiquitin like protein FAT10

产品类型

Monoclonal

检验物种

human, mouse

应用范围

WB, IHC, ICC/IF

基因名称

UBD

克隆号

HID-21

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Ubiquitin D

计算分子量

18 kDa

实际分子量

23 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Ubiquitin D is a protein that in humans is encoded by the UBD gene. It is mapped to 6p22.1. This gene encodes a protein which contains two ubiquitin-like domains and appears to have similar function to ubiquitin. Through covalent attachment,the encoded protein targets other proteins for 26S proteasome degradation. This protein has been implicated to function in many cellular processes,including caspase-dependent apoptosis,formation of aggresomes,mitotic regulation,and dendritic cell maturation. Upregulation of this gene may promote inflammation in chronic kidney disease and has been observed in many cancer types.

Uniprot ID

O15205

文献引用格式

FAT10/UBD Antibody (Clone#HID-21) (Boster Biological Technology, Wuhan, China. Catalog#BM4765)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Ubiquitin D

实验方案>

实验步骤

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