| Western blot (WB): | 1:500-2000 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |

Western blot analysis of Prosurfactant Protein C/SFTPC using anti-Prosurfactant Protein C/SFTPC antibody (A02001). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat lung tissue lysates,
Lane 2: Mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Prosurfactant Protein C/SFTPC antigen affinity purified polyclonal antibody (A02001) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Prosurfactant Protein C/SFTPC at approximately 21 kDa. The expected band size for Prosurfactant Protein C/SFTPC is at 21 kDa.

Western blot analysis of Prosurfactant Protein C/SFTPC using anti-Prosurfactant Protein C/SFTPC antibody (A02001). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat lung tissue lysates,
Lane 2: Mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Prosurfactant Protein C/SFTPC antigen affinity purified polyclonal antibody (A02001) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Prosurfactant Protein C/SFTPC at approximately 21 kDa. The expected band size for Prosurfactant Protein C/SFTPC is at 21 kDa.


