Anti-GPX4 Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

A02059-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of GPX4 using anti-GPX4 antibody (A02059-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 2: MCF-7 whole cell lysates,
Lane 3: COS-7 whole cell lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: rat testis tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GPX4 antigen affinity purified polyclonal antibody (A02059-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GPX4 at approximately 19 kDa. The expected band size for GPX4 is at 22 kDa.
all(3)
Western blot analysis of GPX4 using anti-GPX4 antibody (A02059-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCT116- WT whole cell lysates,
Lane 2: human HCT116-GPX4 KO whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GPX4 antigen affinity purified polyclonal antibody (A02059-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GPX4 at approximately 19 kDa. The expected band size for GPX4 is at 22 kDa.
all(3)
all(3)

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产品简介>

产品名称

Anti-GPX4 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Glutathione peroxidase 4; GPx 4; GPX4; GPx-4; GSHPx 4; GSHPx-4; MCSP; PHGPx; snGPx; snPHGPx

产品类型

Polyclonal

检验物种

human, mouse, rat, monkey

应用范围

WB, ELISA

基因名称

GPX4

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Glutathione Peroxidase 4/GPX4 recombinant protein (Position: A30-F197).

计算分子量

22 kDa

实际分子量

19 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Glutathione peroxidase 4, also known as GPX4, is an enzyme that in humans is encoded by the GPX4 gene. This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants.

Uniprot ID

P36969

RRID

AB_3081513

文献引用格式

GPX4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02059-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

glutathione peroxidase 4

蛋白名全称

Phospholipid hydroperoxide glutathione peroxidase

实验方案>

实验步骤

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