Anti-GPX4 Antibody (Clone#6I4E7)

筛选器： All WB FCM

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  Western blot analysis of GPX4 using anti-GPX4 antibody (M02059-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: HepG2 whole cell lysates,
  Lane 2: Caco-2 whole cell lysates,
  Lane 3: 293T whole cell lysates,
  Lane 4: K562 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-GPX4 antigen affinity purified monoclonal antibody (M02059-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GPX4 at approximately 19 kDa. The expected band size for GPX4 is at 22 kDa.

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  Flow Cytometry analysis of Hela cells using anti-GPX4 antibody (M02059-1).
  Overlay histogram showing Hela cells stained with M02059-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GPX4 Antibody (M02059-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of GPX4 using anti-GPX4 antibody (M02059-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: HepG2 whole cell lysates,
  Lane 2: Caco-2 whole cell lysates,
  Lane 3: 293T whole cell lysates,
  Lane 4: K562 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-GPX4 antigen affinity purified monoclonal antibody (M02059-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GPX4 at approximately 19 kDa. The expected band size for GPX4 is at 22 kDa.

• 

  Flow Cytometry analysis of Hela cells using anti-GPX4 antibody (M02059-1).
  Overlay histogram showing Hela cells stained with M02059-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GPX4 Antibody (M02059-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.