Western blot (WB): | 1:500-2000 |
Western blot analysis of Caspase 6/CASP6 using anti-Caspase 6/CASP6 antibody (A02631). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 6/CASP6 antigen affinity purified polyclonal antibody (A02631) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 6/CASP6 at approximately 33 kDa. The expected band size for Caspase 6/CASP6 is at 33 kDa.
Western blot analysis of Caspase 6/CASP6 using anti-Caspase 6/CASP6 antibody (A02631). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 6/CASP6 antigen affinity purified polyclonal antibody (A02631) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 6/CASP6 at approximately 33 kDa. The expected band size for Caspase 6/CASP6 is at 33 kDa.