Anti-xCT/SLC7A11 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A03036-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of xCT/SLC7A11 using anti-xCT/SLC7A11 antibody (A03036-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-xCT/SLC7A11 antigen affinity purified polyclonal antibody (A03036-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for xCT/SLC7A11 at approximately 40-50 kDa. The expected band size for xCT/SLC7A11 is at 55 kDa.
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产品简介>

产品名称

Anti-xCT/SLC7A11 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Amino acid transport system xc; CCBR1; Cystine/glutamate transporter; SLC7A11; SLC7A11/xCT; xCT

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ELISA

基因名称

SLC7A11

宿主

Rabbit

抗体亚型

IgG

免疫原

Peptide

计算分子量

55 kDa

实际分子量

40-50 kDa

成分

500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

This gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. This protein has been identified as the predominant mediator of Kaposi sarcoma-associated herpesvirus fusion and entry permissiveness into cells. Also, increased expression of this gene in primary gliomas (compared to normal brain tissue) was associated with increased glutamate secretion via the XCT channels, resulting in neuronal cell death.

Uniprot ID

Q9UPY5

文献引用格式

xCT/SLC7A11 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A03036-2)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

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实验步骤

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