Western blot (WB): | 1:500-2000 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
ImmunoPrecipitation (IP): | 1:50 |
Figure 1. Western blot analysis of anti-xCT/SLC7A11 antibody (BM5318). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-xCT/SLC7A11 antigen affinity purified monoclonal antibody (BM5318) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for xCT/SLC7A11 at approximately 55 kDa. The expected band size for xCT/SLC7A11 is at 55 kDa.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.
Figure 1. Western blot analysis of anti-xCT/SLC7A11 antibody (BM5318). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-xCT/SLC7A11 antigen affinity purified monoclonal antibody (BM5318) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for xCT/SLC7A11 at approximately 55 kDa. The expected band size for xCT/SLC7A11 is at 55 kDa.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.
Immunofluorescent analysis using the Antibody.