Anti-GAD65/GAD2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A03142-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti- GAD2 antibody (A03142-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
Use rabbit anti- GAD2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for GAD2 at approximately 65KD. The expected band size for GAD2 is at 65KD.
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IHC analysis of GAD65/GAD2 using anti-GAD65/GAD2 antibody (A03142-1).
GAD65/GAD2 was detected in a paraffin-embedded section of human glioma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GAD65/GAD2 Antibody (A03142-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of GAD65/GAD2 using anti-GAD65/GAD2 antibody (A03142-1).
GAD65/GAD2 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GAD65/GAD2 Antibody (A03142-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
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产品简介>

产品名称

Anti-GAD65/GAD2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

GAD 65; GAD2; GAD65; Glutamate decarboxylase 2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ELISA

基因名称

GAD2

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human GAD65 recombinant protein (Position: K84-L182). Human GAD65 shares 98% and 96% amino acid (aa) sequence identity with mouse and rat GAD65, respectively.

计算分子量

65 kDa

实际分子量

65 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Glutamate decarboxylase 2, also known as GAD65, is an enzyme that in humans is encoded by the GAD2 gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Uniprot ID

Q05329

RRID

AB_3081615

文献引用格式

GAD65/GAD2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A03142-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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