Anti-GAD65/GAD2 Antibody (Clone#4E12)

Mouse monoclonal antibody

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筛选器： All WB

M03142-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of GAD65/GAD2 using anti-GAD65/GAD2 antibody (M03142-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-GAD65/GAD2 antigen affinity purified monoclonal antibody (M03142-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAD65/GAD2 at approximately 65 kDa. The expected band size for GAD65/GAD2 is at 65 kDa.
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产品名称

Anti-GAD65/GAD2 Antibody (Clone#4E12)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

GAD 65; GAD2; GAD65; Glutamate decarboxylase 2

产品类型

Monoclonal

检验物种

mouse, rat

应用范围

基因名称

GAD2

克隆号

4E12

宿主

Mouse

抗体亚型

IgG1

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65, different from the related mouse and rat sequences by one amino acid.

计算分子量

65 kDa

实际分子量

65 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Glutamate decarboxylase 2, also known as GAD65, is an enzyme that in humans is encoded by the GAD2 gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Uniprot ID

Q05329

RRID

AB_3082485

文献引用格式

GAD65/GAD2 Antibody (Clone#4E12) (Boster Biological Technology, Wuhan, China. Catalog#M03142-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)

蛋白名全称

Glutamate decarboxylase 2

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实验步骤

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