| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |

Western blot analysis of KLF10 using anti-KLF10 antibody (A03419-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 2: Jurkat whole cell lysates,
Lane 3: Hela whole cell lysates,
Lane 4: PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-KLF10 antigen affinity purified polyclonal antibody (A03419-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for KLF10 at approximately 53 kDa. The expected band size for KLF10 is at 53 kDa.

ICC/IF analysis of KLF10 using anti-KLF10 antibody (A03419-2).
KLF10 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-KLF10 Antibody (A03419-2) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Western blot analysis of KLF10 using anti-KLF10 antibody (A03419-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 2: Jurkat whole cell lysates,
Lane 3: Hela whole cell lysates,
Lane 4: PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-KLF10 antigen affinity purified polyclonal antibody (A03419-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for KLF10 at approximately 53 kDa. The expected band size for KLF10 is at 53 kDa.

ICC/IF analysis of KLF10 using anti-KLF10 antibody (A03419-2).
KLF10 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-KLF10 Antibody (A03419-2) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).



