Anti-14-3-3 GAMMA/YWHAG-Specific Antibody

筛选器： All WB IHC FCM ELISA

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  Western blot analysis of 14-3-3 GAMMA/YWHAG-Specific using anti-14-3-3 GAMMA/YWHAG-Specific antibody (A04148-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Jurkat whole cell lysates,
  Lane 2: human placenta tissue lysates,
  Lane 3: rat brain tissue lysates,
  Lane 4: rat skeletal muscle tissue lysates,
  Lane 5: mouse brain tissue lysates,
  Lane 6: mouse skeletal muscle tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 GAMMA/YWHAG-Specific antigen affinity purified polyclonal antibody (A04148-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 GAMMA/YWHAG-Specific at approximately 28 kDa. The expected band size for 14-3-3 GAMMA/YWHAG-Specific is at 28 kDa.

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  IHC analysis of 14-3-3 GAMMA/YWHAG-Specific using anti-14-3-3 GAMMA/YWHAG-Specific antibody (A04148-2).14-3-3 GAMMA/YWHAG-Specific was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 GAMMA/YWHAG-Specific Antibody (A04148-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  Flow Cytometry analysis of U87 cells using anti-14-3-3GAMMA/YWHAG-Specific antibody (A04148-2).
  Overlay histogram showing U87 cells stained with A04148-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 GAMMA/YWHAG-Specific Antibody (A04148-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Western blot analysis of 14-3-3 GAMMA/YWHAG-Specific using anti-14-3-3 GAMMA/YWHAG-Specific antibody (A04148-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Jurkat whole cell lysates,
  Lane 2: human placenta tissue lysates,
  Lane 3: rat brain tissue lysates,
  Lane 4: rat skeletal muscle tissue lysates,
  Lane 5: mouse brain tissue lysates,
  Lane 6: mouse skeletal muscle tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 GAMMA/YWHAG-Specific antigen affinity purified polyclonal antibody (A04148-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 GAMMA/YWHAG-Specific at approximately 28 kDa. The expected band size for 14-3-3 GAMMA/YWHAG-Specific is at 28 kDa.

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  IHC analysis of 14-3-3 GAMMA/YWHAG-Specific using anti-14-3-3 GAMMA/YWHAG-Specific antibody (A04148-2).14-3-3 GAMMA/YWHAG-Specific was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 GAMMA/YWHAG-Specific Antibody (A04148-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

• 

  Flow Cytometry analysis of U87 cells using anti-14-3-3GAMMA/YWHAG-Specific antibody (A04148-2).
  Overlay histogram showing U87 cells stained with A04148-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 GAMMA/YWHAG-Specific Antibody (A04148-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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