Western blot analysis of RAB11B using anti-RAB11B antibody (A04526-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat ovary tissue lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse ovary tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse SP20 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB11B antigen affinity purified polyclonal antibody (A04526-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11B at approximately 24 kDa. The expected band size for RAB11B is at 24 kDa.
Western blot analysis of RAB11B using anti-RAB11B antibody (A04526-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human Caco-2 whole cell lysates,
Lane 7: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB11B antigen affinity purified polyclonal antibody (A04526-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11B at approximately 24 kDa. The expected band size for RAB11B is at 24 kDa.
IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
RAB11B was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
RAB11B was detected in a paraffin-embedded section of human ovary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
RAB11B was detected in a paraffin-embedded section of mouse ovary tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
RAB11B was detected in a paraffin-embedded section of rat ovary tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of A549 cells using anti-RAB11B antibody (A04526-1).Overlay histogram showing A549 cells stained with A04526-1 (Blue line).anti-RAB11B Antibody (A04526-1, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(8) 
IF analysis of RAB11B using anti-RAB11B antibody (A04526-1).
RAB11B was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti-RAB11B Antibody (A04526-1) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of RAB11B using anti-RAB11B antibody (A04526-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat ovary tissue lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse ovary tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse SP20 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB11B antigen affinity purified polyclonal antibody (A04526-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11B at approximately 24 kDa. The expected band size for RAB11B is at 24 kDa.
Western blot analysis of RAB11B using anti-RAB11B antibody (A04526-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human Caco-2 whole cell lysates,
Lane 7: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB11B antigen affinity purified polyclonal antibody (A04526-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11B at approximately 24 kDa. The expected band size for RAB11B is at 24 kDa.
IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
RAB11B was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
RAB11B was detected in a paraffin-embedded section of human ovary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
RAB11B was detected in a paraffin-embedded section of mouse ovary tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
RAB11B was detected in a paraffin-embedded section of rat ovary tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of A549 cells using anti-RAB11B antibody (A04526-1).Overlay histogram showing A549 cells stained with A04526-1 (Blue line).anti-RAB11B Antibody (A04526-1, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
IF analysis of RAB11B using anti-RAB11B antibody (A04526-1).
RAB11B was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti-RAB11B Antibody (A04526-1) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Western blot analysis of RAB11B using anti-RAB11B antibody (A04526-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat ovary tissue lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: mouse ovary tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse SP20 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB11B antigen affinity purified polyclonal antibody (A04526-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11B at approximately 24 kDa. The expected band size for RAB11B is at 24 kDa.
Western blot analysis of RAB11B using anti-RAB11B antibody (A04526-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human Caco-2 whole cell lysates,
Lane 7: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB11B antigen affinity purified polyclonal antibody (A04526-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11B at approximately 24 kDa. The expected band size for RAB11B is at 24 kDa.
IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
RAB11B was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
RAB11B was detected in a paraffin-embedded section of human ovary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
RAB11B was detected in a paraffin-embedded section of mouse ovary tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
RAB11B was detected in a paraffin-embedded section of rat ovary tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of A549 cells using anti-RAB11B antibody (A04526-1).Overlay histogram showing A549 cells stained with A04526-1 (Blue line).anti-RAB11B Antibody (A04526-1, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
IF analysis of RAB11B using anti-RAB11B antibody (A04526-1).
RAB11B was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti-RAB11B Antibody (A04526-1) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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