Anti-RAB11B Antibody (Clone#6C5)

筛选器： All WB FCM ICC/IF

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  Western blot analysis of RAB11B using anti-RAB11B antibody (M04526). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HEK293 tissue lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human A549 whole cell lysates,
  Lane 4: human placenta whole cell lysates,
  Lane 5: human HepG2 whole cell lysates,
  Lane 6: human Caco-2 whole cell lysates,
  Lane 7: human THP-1 whole cell lysates,
  Lane 8: human Raji whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-RAB11B antigen affinity purified monoclonal antibody (M04526) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11B at approximately 24 kDa. The expected band size for RAB11B is at 24 kDa.

• 

  Western blot analysis of RAB11B using anti-RAB11B antibody (M04526). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates,
  Lane 2: rat lung whole cell lysates,
  Lane 3: rat spleen whole cell lysates,
  Lane 4: rat C6 whole cell lysates,
  Lane 5: mouse brain whole cell lysates,
  Lane 6: mouse lung whole cell lysates,
  Lane 7: mouse spleen whole cell lysates,
  Lane 8: mouse Neuro-2a whole cell lysates,
  Lane 9: mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-RAB11B antigen affinity purified monoclonal antibody (M04526) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11B at approximately 24 kDa. The expected band size for RAB11B is at 24 kDa.

• 

  Flow Cytometry analysis of Caco-2 cells using anti-RAB11B antibody (M04526).
  Overlay histogram showing Caco-2 cells stained with M04526 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RAB11B Antibody (M04526) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  ICC/IF analysis of RAB11B using anti-RAB11B antibody (M04526).
  RAB11B was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with mouse anti-RAB11B Antibody (M04526) at a dilution of 1:100. Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Western blot analysis of RAB11B using anti-RAB11B antibody (M04526). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HEK293 tissue lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human A549 whole cell lysates,
  Lane 4: human placenta whole cell lysates,
  Lane 5: human HepG2 whole cell lysates,
  Lane 6: human Caco-2 whole cell lysates,
  Lane 7: human THP-1 whole cell lysates,
  Lane 8: human Raji whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-RAB11B antigen affinity purified monoclonal antibody (M04526) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11B at approximately 24 kDa. The expected band size for RAB11B is at 24 kDa.

• 

  Western blot analysis of RAB11B using anti-RAB11B antibody (M04526). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates,
  Lane 2: rat lung whole cell lysates,
  Lane 3: rat spleen whole cell lysates,
  Lane 4: rat C6 whole cell lysates,
  Lane 5: mouse brain whole cell lysates,
  Lane 6: mouse lung whole cell lysates,
  Lane 7: mouse spleen whole cell lysates,
  Lane 8: mouse Neuro-2a whole cell lysates,
  Lane 9: mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-RAB11B antigen affinity purified monoclonal antibody (M04526) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11B at approximately 24 kDa. The expected band size for RAB11B is at 24 kDa.

• 

  Flow Cytometry analysis of Caco-2 cells using anti-RAB11B antibody (M04526).
  Overlay histogram showing Caco-2 cells stained with M04526 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RAB11B Antibody (M04526) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  ICC/IF analysis of RAB11B using anti-RAB11B antibody (M04526).
  RAB11B was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with mouse anti-RAB11B Antibody (M04526) at a dilution of 1:100. Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).