Anti-CD3 Gamma/CD3G Antibody
送常用试剂

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM ELISA

A04853-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-CD3 Gamma/CD3G antibody (A04853-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOMLT-4 whole cell lysates,
Lane 2: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD3 Gamma/CD3G antigen affinity purified polyclonal antibody (A04853-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD3 Gamma/CD3G at approximately 20-25 kDa. The expected band size for CD3 Gamma/CD3G is at 20-25 kDa.
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IHC analysis of CD3 Gamma/CD3G using anti-CD3 Gamma/CD3G antibody (A04853-2).
CD3 Gamma/CD3G was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-CD3 Gamma/CD3G Antibody (A04853-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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ICC/IF analysis of CD3 Gamma/CD3G using anti-CD3 Gamma/CD3G antibody (A04853-2).
CD3 Gamma/CD3G was detected in an immunocytochemical section of Jurkat cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of Jurkat cells using anti-CD3 Gamma/CD3G antibody (A04853-2).
Overlay histogram showing Jurkat cells stained with A04853-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD3 Gamma/CD3G Antibody (A04853-2, 1:100). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品简介>

产品名称

Anti-CD3 Gamma/CD3G Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CD3 gamma, CD3G, CD3-gamma, T cell receptor T3 gamma chain, T3G

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, FCM, ELISA

基因名称

CD3G

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human CD3 Gamma/CD3G recombinant protein (Position: Q23-D165). Human CD3G shares 67.8% and 70.6% amino acid (aa) sequence identity with mouse and rat CD3G, respectively.

计算分子量

20 kDa

实际分子量

20 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

T-cell surface glycoprotein CD3 gamma chain is a protein that in humans is encoded by the CD3G gene. The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency.

Uniprot ID

P09693

Gene ID

917

文献引用格式

CD3 Gamma/CD3G Antibody (Boster Biological Technology, Wuhan, China. Catalog#A04853-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD3 gamma subunit of T-cell receptor complex

蛋白名全称

T-cell surface glycoprotein CD3 gamma chain

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