Western blot analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GM130/GOLGA2 antigen affinity purified polyclonal antibody (A05865-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GM130/GOLGA2 at approximately 130 kDa. The expected band size for GM130/GOLGA2 is at 113 kDa.
IHC analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1).
GM130/GOLGA2 was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GM130/GOLGA2 Antibody (A05865-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1).
GM130/GOLGA2 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GM130/GOLGA2 Antibody (A05865-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1).
GM130/GOLGA2 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GM130/GOLGA2 Antibody (A05865-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1).
GM130/GOLGA2 was detected in frozen section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GM130/GOLGA2 Antibody (A05865-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1).
GM130/GOLGA2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-GM130/GOLGA2 Antibody (A05865-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IP analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1) in U251 whole cell lysate.
Western blot analysis of GM130/GOLGA2 using anti- GM130/GOLGA2 antibody (A05865-1).
Lane 1: HepG2 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- GM130/GOLGA2 antibody in HepG2 whole cell lysate,
Lane 3: anti- GM130/GOLGA2 antibody (2μg) + HepG2 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- GM130/GOLGA2 antigen affinity purified polyclonal antibody (A05865-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GM130/GOLGA2 at approximately 150 kDa. The expected band size for GM130/GOLGA2 is at 113 kDa.
Flow Cytometry analysis of PC-3 cells using anti- GM130 antibody (A05865-1).
Overlay histogram showing PC-3 cells stained with A05865-1 (Blue line). anti-GM130 Antibody (A05865-1, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of U2OS cells using anti- GM130 antibody (A05865-1).
Overlay histogram showing U2OS cells stained with A05865-1 (Blue line). anti-GM130 Antibody (A05865-1, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| ImmunoPrecipitation (IP): | 1:200-300 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GM130/GOLGA2 antigen affinity purified polyclonal antibody (A05865-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GM130/GOLGA2 at approximately 130 kDa. The expected band size for GM130/GOLGA2 is at 113 kDa.
IHC analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1).
GM130/GOLGA2 was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GM130/GOLGA2 Antibody (A05865-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1).
GM130/GOLGA2 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GM130/GOLGA2 Antibody (A05865-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1).
GM130/GOLGA2 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GM130/GOLGA2 Antibody (A05865-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1).
GM130/GOLGA2 was detected in frozen section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GM130/GOLGA2 Antibody (A05865-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1).
GM130/GOLGA2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-GM130/GOLGA2 Antibody (A05865-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IP analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1) in U251 whole cell lysate.
Western blot analysis of GM130/GOLGA2 using anti- GM130/GOLGA2 antibody (A05865-1).
Lane 1: HepG2 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- GM130/GOLGA2 antibody in HepG2 whole cell lysate,
Lane 3: anti- GM130/GOLGA2 antibody (2μg) + HepG2 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- GM130/GOLGA2 antigen affinity purified polyclonal antibody (A05865-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GM130/GOLGA2 at approximately 150 kDa. The expected band size for GM130/GOLGA2 is at 113 kDa.
Flow Cytometry analysis of PC-3 cells using anti- GM130 antibody (A05865-1).
Overlay histogram showing PC-3 cells stained with A05865-1 (Blue line). anti-GM130 Antibody (A05865-1, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of U2OS cells using anti- GM130 antibody (A05865-1).
Overlay histogram showing U2OS cells stained with A05865-1 (Blue line). anti-GM130 Antibody (A05865-1, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Western blot analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GM130/GOLGA2 antigen affinity purified polyclonal antibody (A05865-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GM130/GOLGA2 at approximately 130 kDa. The expected band size for GM130/GOLGA2 is at 113 kDa.
IHC analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1).
GM130/GOLGA2 was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GM130/GOLGA2 Antibody (A05865-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1).
GM130/GOLGA2 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GM130/GOLGA2 Antibody (A05865-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1).
GM130/GOLGA2 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GM130/GOLGA2 Antibody (A05865-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1).
GM130/GOLGA2 was detected in frozen section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GM130/GOLGA2 Antibody (A05865-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1).
GM130/GOLGA2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-GM130/GOLGA2 Antibody (A05865-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IP analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (A05865-1) in U251 whole cell lysate.
Western blot analysis of GM130/GOLGA2 using anti- GM130/GOLGA2 antibody (A05865-1).
Lane 1: HepG2 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- GM130/GOLGA2 antibody in HepG2 whole cell lysate,
Lane 3: anti- GM130/GOLGA2 antibody (2μg) + HepG2 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- GM130/GOLGA2 antigen affinity purified polyclonal antibody (A05865-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GM130/GOLGA2 at approximately 150 kDa. The expected band size for GM130/GOLGA2 is at 113 kDa.
Flow Cytometry analysis of PC-3 cells using anti- GM130 antibody (A05865-1).
Overlay histogram showing PC-3 cells stained with A05865-1 (Blue line). anti-GM130 Antibody (A05865-1, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of U2OS cells using anti- GM130 antibody (A05865-1).
Overlay histogram showing U2OS cells stained with A05865-1 (Blue line). anti-GM130 Antibody (A05865-1, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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