Anti-GM130/GOLGA2 Antibody (Clone#6D4)

Mouse monoclonal antibody

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筛选器： All WB IHC FCM ICC/IF

M05865-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (M05865-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Human placenta tissue lysates,
Lane 2: Hela whole cell lysates,
Lane 3: K562 whole cell lysates,
Lane 4: Caco-2 whole cell lysates,
Lane 5: A549 whole cell lysates,
Lane 6: A431 whole cell lysates,
Lane 7: HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-GM130/GOLGA2 antigen affinity purified monoclonal antibody (M05865-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GM130/GOLGA2 at approximately 130 kDa. The expected band size for GM130/GOLGA2 is at 113 kDa.
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Western blot analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (M05865-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Rat liver tissue lysates,
Lane 3: Mouse brain tissue lysates,
Lane 4: Mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-GM130/GOLGA2 antigen affinity purified monoclonal antibody (M05865-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GM130/GOLGA2 at approximately 130 kDa. The expected band size for GM130/GOLGA2 is at 113 kDa.
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IHC analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (M05865-2).
GM130/GOLGA2 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-GM130/GOLGA2 Antibody (M05865-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (M05865-2).
GM130/GOLGA2 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-GM130/GOLGA2 Antibody (M05865-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (M05865-2).
GM130/GOLGA2 was detected in a paraffin-embedded section of rat pancreas tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-GM130/GOLGA2 Antibody (M05865-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
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Flow cytometry analysis of A431 cell(1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).
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IF analysis of GM130/GOLGA2 using anti-GM130/GOLGA2 antibody (M05865-2).
GM130/GOLGA2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with mouse anti-GM130/GOLGA2 Antibody (M05865-2) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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产品简介>

产品名称

Anti-GM130/GOLGA2 Antibody (Clone#6D4)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cell and organelle markers; GM130; GM130 autoantigen; GOLGA2; Golgi Marker; Golgin 95; Golgin subfamily A member 2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM, ICC/IF

基因名称

GOLGA2

克隆号

6D4

宿主

Mouse

抗体亚型

IgG1

免疫原

E. coli-derived human GM130 recombinant protein (Position: E796-E913). Human GM130 shares 82.9% amino acid (aa) sequence identity with both mouse and rat GM130.

计算分子量

113 kDa

实际分子量

130 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Golgin subfamily A member 2 is a protein that in humans is encoded by the GOLGA2 gene. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.

Uniprot ID

Q08379

RRID

AB_3082496

文献引用格式

GM130/GOLGA2 Antibody (Clone#6D4) (Boster Biological Technology, Wuhan, China. Catalog#M05865-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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