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Anti-MFSD12 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ELISA

A16855-1

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti-MFSD12 antibody (A16855-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates,
    Lane 2: human SiHa whole cell lysates,
    Lane 3: human A549 whole cell lysates,
    Lane 4: human PC-3 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MFSD12 antigen affinity purified polyclonal antibody (A16855-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MFSD12 at approximately 52 kDa. The expected band size for MFSD12 is at 52 kDa.

    all(4)
  • IHC analysis of MFSD12 using anti-MFSD12 antibody (A16855-1).
    MFSD12 was detected in a paraffin-embedded section of human testicular seminoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(4)
  • IHC analysis of MFSD12 using anti-MFSD12 antibody (A16855-1).
    MFSD12 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(4)

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产品名称
Anti-MFSD12 Antibody
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
MFSD12; C19orf28; Major facilitator superfamily domain-containing protein 12
产品类型
Polyclonal
检验物种
human
应用范围
WB, IHC, ELISA
基因名称
MFSD12
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived human MFSD12 recombinant protein (Position: Q66-H443). Human MFSD12 shares 86.8% amino acid (aa) sequence identity with mouse MFSD12.
计算分子量
52 kDa
实际分子量
52 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
The major facilitator superfamily consists of presumed carbohydrate transporters with 10-12 membrane-spanning domains. MFSD12 (major facilitator superfamily domain containing 12), also known as PP3501 or C19orf28, is a 480 amino acid multi-pass membrane protein that belongs to the major facilitator superfamily. Existing as two alternatively spliced isoforms, MFSD12 is encoded by a gene that maps to human chromosome 19p13.3. Chromosome 19 consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin (Ig) superfamily members, including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG families, and Fc receptors (FcRs).
Uniprot ID
Q6NUT3  
Gene ID
126321  
文献引用格式
MFSD12 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A16855-1)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
基因名全称
major facilitator superfamily domain containing 12
蛋白名全称
Major facilitator superfamily domain-containing protein 12
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Enzyme linked immunosorbent assay (ELISA):1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-MFSD12 Antibody

    筛选器: All WB IHC ELISA

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