Anti-MKL1/MRTFA Antibody

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF ELISA

A32468-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of MKL1/MRTFA using anti-MKL1/MRTFA antibody (A32468-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MKL1/MRTFA antigen affinity purified polyclonal antibody (A32468-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MKL1/MRTFA at approximately 145 kDa. The expected band size for MKL1/MRTFA is at 99 kDa.
all(3)
IF analysis of MKL1/MRTFA using anti-MKL1/MRTFA antibody (A32468-2).
MKL1/MRTFA was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-MKL1/MRTFA Antibody (A32468-2) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(3)
all(3)

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产品简介>

产品名称

Anti-MKL1/MRTFA Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

BSAC; KIAA1438; MAL; MKL/myocardin like protein 1; MKL1; MRTF A; MRTFA; MRTF-A

产品类型

Polyclonal

检验物种

human

应用范围

WB, ICC/IF, ELISA

基因名称

MRTFA

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human Mkl1/MRTFA recombinant protein (Position: L192-L931).

计算分子量

99 kDa

实际分子量

145 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants.

Uniprot ID

Q969V6

文献引用格式

MKL1/MRTFA Antibody (Boster Biological Technology, Wuhan, China. Catalog#A32468-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

myocardin related transcription factor A

蛋白名全称

Myocardin-related transcription factor A

实验方案>

实验步骤

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