Anti-F8 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC

BA0046

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of F8 using anti-F8 antibody (BA0046). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: human Hela whole cell lysates,
Lane 7: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-F8 antigen affinity purified polyclonal antibody (BA0046) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for F8 at approximately 92 kDa. The expected band size for F8 is at 267 kDa.
all(2)
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-F8 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AHF; Antihemophilic factor; Coagulation factor VIII; DXS1253E; F8; F8B; F8C; FVIII; HEMA; Procoagulant component

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human Factor VIII.

计算分子量

267 kDa

实际分子量

92 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Coagulation Factor VIII(FVIII) is an essential blood-clotting protein, also known as anti-hemophilic factor(AHF). By in situ hybridization, Tantravahi et al.(1986) concluded that the F8 gene is located in the proximal part of chromosome Xq28 with probes DX13 and St14 distally located. The F8 gene encodes coagulation factor VIII, a large plasma glycoprotein that functions in the blood coagulation cascade as a cofactor for the factor IXa-dependent activation of factor X(F10). Factor VIII is activated proteolytically by a variety of coagulation enzymes, including thrombin(F2). Factor VIII is tightly associated in the blood with von Willebrand factor(VWF), which serves as a protective carrier protein for factor VIII(Toole et al., 1984; Hoyer, 1994).

Uniprot ID

P00451

文献引用格式

F8 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0046)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

coagulation factor VIII, procoagulant component

蛋白名全称

Coagulation factor VIII

实验方案>

实验步骤

引用文献(0)>

清除

Anti-F8 Antibody

筛选器： All WB IHC

Western blot analysis of F8 using anti-F8 antibody (BA0046). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: human Hela whole cell lysates,
Lane 7: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-F8 antigen affinity purified polyclonal antibody (BA0046) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for F8 at approximately 92 kDa. The expected band size for F8 is at 267 kDa.

亮暗模式切换

Western blot (WB):	1:500-2000
Immunohistochemistry (IHC):	1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Anti-F8 Antibody

筛选器： All WB IHC

BA0046

产品简介>

产品名称

规格/价格

指标别称

产品类型

检验物种

应用范围

基因名称

宿主

抗体亚型

免疫原

计算分子量

实际分子量

成分

纯化方式

浓度

产品形态

保存条件

背景资料

Uniprot ID

文献引用格式

应用释义

推荐配套的二抗和检测试剂

基因名全称

蛋白名全称

推荐稀释比

实验方案>

引用文献(0)>

All IHC(12) ICC(3) WB(2) IF(2) All Species Human(7) Rat(5) Mouse(2) Rabbit(2) Dog(1) 清除

相关产品>

BA0046-2

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Anti-F8 Antibody

筛选器： All WB IHC

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