Western blot (WB): | 1:500-2000 |
Western blot analysis of F8 using anti-F8 antibody (BA0046-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: human Hela whole cell lysates,
Lane 7: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-F8 antigen affinity purified polyclonal antibody (BA0046-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for F8 at approximately 92 kDa. The expected band size for F8 is at 267 kDa.
Western blot analysis of F8 using anti-F8 antibody (BA0046-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: human Hela whole cell lysates,
Lane 7: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-F8 antigen affinity purified polyclonal antibody (BA0046-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for F8 at approximately 92 kDa. The expected band size for F8 is at 267 kDa.