Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Western blot analysis of TGF Beta 1/TGFB1 using anti-TGF Beta 1/TGFB1 antibody (BA0290). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Smmc whole cell lysates,
Lane 2: RH35 whole cell lysates,
Lane 3: HepA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TGF Beta 1/TGFB1 antigen affinity purified polyclonal antibody (BA0290) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TGF Beta 1/TGFB1 at approximately 14 kDa/48 kDa. The expected band size for TGF Beta 1/TGFB1 is at 44 kDa.
IHC analysis of TGF Beta 1/TGFB1 using anti-TGF Beta 1/TGFB1 antibody (BA0290).
TGF Beta 1/TGFB1 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-TGF Beta 1/TGFB1 Antibody (BA0290) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of TGF Beta 1/TGFB1 using anti-TGF Beta 1/TGFB1 antibody (BA0290). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Smmc whole cell lysates,
Lane 2: RH35 whole cell lysates,
Lane 3: HepA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TGF Beta 1/TGFB1 antigen affinity purified polyclonal antibody (BA0290) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TGF Beta 1/TGFB1 at approximately 14 kDa/48 kDa. The expected band size for TGF Beta 1/TGFB1 is at 44 kDa.
IHC analysis of TGF Beta 1/TGFB1 using anti-TGF Beta 1/TGFB1 antibody (BA0290).
TGF Beta 1/TGFB1 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-TGF Beta 1/TGFB1 Antibody (BA0290) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.