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Anti-TGF Beta 1/TGFB1 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

BA0290

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of TGF Beta 1/TGFB1 using anti-TGF Beta 1/TGFB1 antibody (BA0290). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: Smmc whole cell lysates,
    Lane 2: RH35 whole cell lysates,
    Lane 3: HepA whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TGF Beta 1/TGFB1 antigen affinity purified polyclonal antibody (BA0290) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TGF Beta 1/TGFB1 at approximately 14 kDa/48 kDa. The expected band size for TGF Beta 1/TGFB1 is at 44 kDa.

    all(2)
  • IHC analysis of TGF Beta 1/TGFB1 using anti-TGF Beta 1/TGFB1 antibody (BA0290).
    TGF Beta 1/TGFB1 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-TGF Beta 1/TGFB1 Antibody (BA0290) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(2)

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产品名称
Anti-TGF Beta 1/TGFB1 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CED; DPD1; LAP; TGF beta 1; tgf beta1; TGFB; TGFB1; TGFbeta; TGF-beta 1; tgfbeta1; tgf-beta1; TGFβ; TGF-β; TGFβ1; TGF-β1
产品类型
Polyclonal
检验物种
human, mouse, rat, rabbit
应用范围
WB, IHC
基因名称
TGFB1
宿主
Rabbit
抗体亚型
IgG
免疫原
Polypeptide
计算分子量
44 kDa
实际分子量
14 kDa/48 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
Uniprot ID
P01137  
文献引用格式
TGF Beta 1/TGFB1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0290)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
transforming growth factor beta 1
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-TGF Beta 1/TGFB1 Antibody

    筛选器: All WB IHC

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