| Western blot (WB): | 1:500-2000 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |

Western blot analysis of TIMP3 using anti-TIMP3 antibody (BA0577-2).
Lane 1: recombinant human TIMP3 protein 10ng,
Lane 2: recombinant human TIMP3 protein 5ng,
Lane 3: recombinant human TIMP3 protein 2.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TIMP3 antigen affinity purified polyclonal antibody (BA0577-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TIMP3 at approximately 24 kDa.

Western blot analysis of TIMP3 using anti-TIMP3 antibody (BA0577-2).
Lane 1: recombinant human TIMP3 protein 10ng,
Lane 2: recombinant human TIMP3 protein 5ng,
Lane 3: recombinant human TIMP3 protein 2.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TIMP3 antigen affinity purified polyclonal antibody (BA0577-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TIMP3 at approximately 24 kDa.


