Anti-Gelsolin/GSN Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BA0891

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (BA0891). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Gelsolin/GSN antigen affinity purified polyclonal antibody (BA0891) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Gelsolin/GSN at approximately 86 kDa. The expected band size for Gelsolin/GSN is at 86 kDa.
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IHC analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (BA0891).
Gelsolin/GSN was detected in a paraffin-embedded section of Rat Cardiac Muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Gelsolin/GSN Antibody (BA0891) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (BA0891).
Gelsolin/GSN was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Gelsolin/GSN Antibody (BA0891) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (BA0891).
Gelsolin/GSN was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Gelsolin/GSN Antibody (BA0891) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (BA0891).
Gelsolin/GSN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Gelsolin/GSN Antibody (BA0891) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of U87 cells using anti-Gelsolin/GSN antibody (BA0891).
Overlay histogram showing U87 cells stained with BA0891 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gelsolin/GSN Antibody (BA0891) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Gelsolin/GSN Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Actin depolymerizing factor; ADF; AGEL; Brevin; Gelsolin; GSN

产品类型

Polyclonal

检验物种

human, mouse, rat, monkey

应用范围

WB, IHC, ICC/IF, FCM

基因名称

GSN

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Gelsolin, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

计算分子量

86 kDa

实际分子量

86 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Gelsolin also known as GNS is an actin-binding protein that is a key regulator of actin filament assembly and disassembly. Gelsolin is one of the most potent members of the actin-severing gelsolin/villin superfamily. The gene was assigned to human chromosome 9q33.2. Gelsolin is also known as brevin, or actin-depolymerizing factor; it is the principal intracellular and extracellular actin-severing protein. Gelsolin and Gc protein together constitute the extracellular actin-scavenger system which prevents the toxic effects of actin release into the extracellular space under circumstances of cell necrosis. Gelsolin may have therapeutic potential as a mucolytic agent in CF patients. The antiapoptotic activity of gelsolin seems to prevent a step leading to cytochrome c release from the mitochondria into the cytosol.

Uniprot ID

P06396

文献引用格式

Gelsolin/GSN Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0891)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

gelsolin

蛋白名全称

Gelsolin

实验方案>

实验步骤

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