Anti-Gelsolin/GSN Antibody (Clone#GS-2C4)

筛选器： All WB IHC

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  Western blot analysis of Gelsolin using anti-Gelsolin antibody (BM1620).
  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  
  Lane 1: human Hela whole cell lysate,
  
  Lane 2: human A549 whole cell lysate,
  
  Lane 3: monkey COS-7 whole cell lysate,
  
  Lane 4: human Caco-2 whole cell lysate,
  
  Lane 5: human HepG2 whole cell lysate.
  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Gelsolin antigen affinity purified monoclonal antibody (Catalog # BM1620) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Gelsolin at approximately 90KD. The expected band size for Gelsolin is at 86KD.

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  Western blot analysis of Gelsolin using anti-Gelsolin antibody (BM1620).
  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  
  Lane 1: human Hela whole cell lysate,
  
  Lane 2: human A549 whole cell lysate,
  
  Lane 3: monkey COS-7 whole cell lysate,
  
  Lane 4: human Caco-2 whole cell lysate,
  
  Lane 5: human HepG2 whole cell lysate.
  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Gelsolin antigen affinity purified monoclonal antibody (Catalog # BM1620) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Gelsolin at approximately 90KD. The expected band size for Gelsolin is at 86KD.

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