Western blot analysis of anti-Gelsolin/GSN antibody (PB9209). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human COS-7 whole cell lysates,
Lane 5: monkey kidney tissue lysates,
Lane 6: human Caco-2 whole cell lysates,
Lane 7: rat PC-12 whole cell lysates,
Lane 8: mouse RAW246.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Gelsolin/GSN antigen affinity purified polyclonal antibody (PB9209) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Gelsolin/GSN at approximately 86 kDa. The expected band size for Gelsolin/GSN is at 86 kDa.
IHC analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (PB9209).
Gelsolin/GSN was detected in a paraffin-embedded section of intestinal cancer tissue. The tissue section was incubated with rabbit anti-Gelsolin/GSN Antibody (PB9209) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (PB9209).
Gelsolin/GSN was detected in a paraffin-embedded section of lung cancer tissue. The tissue section was incubated with rabbit anti-Gelsolin/GSN Antibody (PB9209) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (PB9209).
Gelsolin/GSN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Gelsolin/GSN Antibody (PB9209) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow cytometry analysis of THP-1 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(5) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of anti-Gelsolin/GSN antibody (PB9209). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human COS-7 whole cell lysates,
Lane 5: monkey kidney tissue lysates,
Lane 6: human Caco-2 whole cell lysates,
Lane 7: rat PC-12 whole cell lysates,
Lane 8: mouse RAW246.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Gelsolin/GSN antigen affinity purified polyclonal antibody (PB9209) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Gelsolin/GSN at approximately 86 kDa. The expected band size for Gelsolin/GSN is at 86 kDa.
IHC analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (PB9209).
Gelsolin/GSN was detected in a paraffin-embedded section of intestinal cancer tissue. The tissue section was incubated with rabbit anti-Gelsolin/GSN Antibody (PB9209) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (PB9209).
Gelsolin/GSN was detected in a paraffin-embedded section of lung cancer tissue. The tissue section was incubated with rabbit anti-Gelsolin/GSN Antibody (PB9209) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (PB9209).
Gelsolin/GSN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Gelsolin/GSN Antibody (PB9209) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow cytometry analysis of THP-1 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Western blot analysis of anti-Gelsolin/GSN antibody (PB9209). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human COS-7 whole cell lysates,
Lane 5: monkey kidney tissue lysates,
Lane 6: human Caco-2 whole cell lysates,
Lane 7: rat PC-12 whole cell lysates,
Lane 8: mouse RAW246.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Gelsolin/GSN antigen affinity purified polyclonal antibody (PB9209) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Gelsolin/GSN at approximately 86 kDa. The expected band size for Gelsolin/GSN is at 86 kDa.
IHC analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (PB9209).
Gelsolin/GSN was detected in a paraffin-embedded section of intestinal cancer tissue. The tissue section was incubated with rabbit anti-Gelsolin/GSN Antibody (PB9209) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (PB9209).
Gelsolin/GSN was detected in a paraffin-embedded section of lung cancer tissue. The tissue section was incubated with rabbit anti-Gelsolin/GSN Antibody (PB9209) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (PB9209).
Gelsolin/GSN was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Gelsolin/GSN Antibody (PB9209) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Flow cytometry analysis of THP-1 cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
联系我们027-67845390
关注我们
本司产品仅用于科研，不用于临床诊断和治疗
联系方式：027-67845390/1/2 技术支持：武汉丰网
© 1993-2025 Boster Biological Technology co.Itd E-mail:boster@boster.com
鄂ICP备05005548号-2
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
