Anti-Lamin B1/LMNB1 Antibody

Rabbit Polyclonal Antibody

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筛选器： All WB IHC ICC/IF

BA1228

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Lamin B1/LMNB1 using anti-Lamin B1/LMNB1 antibody (BA1228). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human THP-1 whole cell lvsates,
Lane 3: human HL-60 whole cell lvsates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Lamin B1/LMNB1 antigen affinity purified polyclonal antibody (BA1228) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Lamin B1/LMNB1 at approximately 66-72 kDa. The expected band size for Lamin B1/LMNB1 is at 66 kDa.
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IHC analysis of Lamin B1/LMNB1 using anti-Lamin B1/LMNB1 antibody (BA1228).
Lamin B1/LMNB1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lamin B1/LMNB1 Antibody (BA1228) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of Lamin B1/LMNB1 using anti-Lamin B1/LMNB1 antibody (BA1228).
Lamin B1/LMNB1 was detected in frozen section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Lamin B1/LMNB1 Antibody (BA1228) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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ICC analysis of LMNB1 using anti- LMNB1 antibody (BA1228).
LMNB1 was detected in an immunocytochemical section of A549 cells. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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IF analysis of Lamin B1/LMNB1 using anti-Lamin B1/LMNB1 antibody (BA1228) and anti-Alpha Tubulin antibody (M03989-3).
Lamin B1/LMNB1 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-Lamin B1/LMNB1 Antibody (BA1228) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(6)
IF analysis of Lamin B1/LMNB1 using anti-Lamin B1/LMNB1 antibody (BA1228).
Lamin B1/LMNB1 was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-Lamin B1/LMNB1 Antibody (BA1228) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(6)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Lamin B1/LMNB1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ADLD; lamin B1; LMN; LMN2; LMNB; LMNB1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

LMNB1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B1, different from the related rat sequence by one amino acid, and from the related mouse sequence by three amino acids.

计算分子量

66 kDa

实际分子量

66-72 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are though to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Uniprot ID

P20700

文献引用格式

Lamin B1/LMNB1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1228)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

lamin B1

实验方案>

实验步骤

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